Problem 9.8

a) Electrospray ionization is well known for creating multiply charged analyte ions, e.g., multiply protonated [M+nH]n+ species. Do you expect doubly or triply protonated molecules to be suitable for MS/MS? Will they undergo fragmentation easier than singly charged ions or won’t they work at all?

b) Which method of ion dissociation depends on the presence of multiply charged precursor ions?

c) Consider a [M+2H]2+ peptide ion that is subjected to dissociation. Explain which m/z range you are going to scan and why you decided to do so.

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